Visual OligoResearch-grade oligonucleotide tools
DNA/RNA sequence to Tm and 3D
GC content, melting temperature, molecular weight, and double-strand 3D—for oligo design and QC.
Research and education only. Not medical advice.
Go to Oligo Properties (MW/Tm) to get started.
How it works
Search or enter sequence
Use the search above or go to the calculator to enter DNA/RNA sequence.Peptide Properties
Get properties & dose
Get GC%, Tm, molecular weight, charge, secondary structure.
Verify purity
View 3D double-strand and export FASTA, PDB, PNG.Open Verify →
Sequence → Tm & properties
Enter DNA or RNA sequence. Get GC%, Tm, molecular weight, charge, secondary structure. 3D viewer and export FASTA, PDB, PNG.
3D Structure Viewer — DNA/RNA
View DNA/RNA and oligonucleotide structures in-site (rotate, zoom, measure, export). Load by PDB ID or upload.
Calculator · 3D Viewer · Export
Oligo properties (MW/Tm), GC content calculator, salt-adjusted Tm; DNA/RNA 3D structure, double helix builder, PDB upload; export formats and batch analysis export. Calculator → Tools →
Oligo Properties (MW/Tm/GC)
DNA/RNA sequence → MW, Tm, GC%, primer pair Tm, charge.
Salt Adjusted Tm
Tm with salt concentration; molarity and dilution.
DNA/RNA 3D Structure
View DNA/RNA 3D structure. Load PDB, measure, export.
Double Helix Builder
Multi-frame 3D and double-helix preview.
PDB Upload
Load oligonucleotide or nucleic acid PDB. View, export.
Export Formats
FASTA, PDB, PNG. Download and cite.
Batch Analysis Export
Export multiple oligo analyses (table, CSV).
Docs
Getting started, Tm calculation, data sources, tutorials.
Pricing
Free tier for research. Pro & Enterprise for teams and API.
Calculation standards & reproducibility — IUPAC, Bjellqvist (pI), Expasy ProtParam. All results traceable and citable.
For peptide purity verification (Janoshik), see Verify. For Visual Oligo quality control, use your preferred third-party lab.