Salt Adjusted Tm

Convert between mass (mg) and amount (nmol, μmol). Compute stock concentration and dilution volumes. Enter molecular weight (from Oligo Properties or manually) for oligo resuspension and primer dilutions.

Research use only. Not for diagnostic or therapeutic use.

Molecular weight (Da)

MW (Da)

Mass ↔ Amount

mg → nmol / μmol

Mass (mg)

= 1000.00 nmol · 1.0000 μmol

nmol / μmol → mg

Amount (nmol)Amount (μmol)

nmol → 1.0000 mg · μmol → 1.0000 mg

Stock solution

Mass and volume → concentration

Mass (mg)Volume (mL)

Concentration: 1.0000 mg/mL · 1.0000 mM · 1000.00 μM

Dilution

From stock (μM) to target concentration and volume

Stock conc. (μM)(from above: 1000.00)Target conc. (μM)Target volume (mL)

Volume of stock needed: 0.0100 mL

Oligo Properties (MW from sequence) · DNA/RNA 3D Structure · Export formats · Tm Calculation