Oligonucleotide Properties

Enter a DNA or RNA sequence (5′→3′) to get molecular weight, GC%, Tm (melting temperature), and ε260. Fetch sequences by GenBank/RefSeq accession or use the primer pair section for forward/reverse Tm and suggested annealing. Download a PDF report for lab or publication; use Salt Adjusted Tm for molarity and dilution.

Research use only. Tm: simplified Wallace rule with optional salt correction.

Sequence

TypeSequence

Or fetch by GenBank/RefSeq accession:

Primer pair (Tm match)

Enter forward and reverse primer sequences (5′→3′) to compare Tm and get a suggested annealing temperature.

Forward (5′→3′)Reverse (5′→3′)

Salt Adjusted Tm · DNA/RNA 3D Structure · Double Helix Builder · Export formats · Batch Analysis Export · Tm Calculation